Convert CSV and TSV files containing sequence data to FASTA format with flexible column mapping and automatic delimiter detection
Convert FASTA sequence files to FASTQ format with mock quality scores
Convert standard FASTQ reads to FASTA with validation, IUPAC nucleotide support, average-quality filtering, and downloadable summaries
Convert GenBank files to FASTA format
Convert RNA sequences to DNA (reverse transcription) - replaces U with T
Convert TXT or plain text sequences into FASTA format files for DNA, RNA, and protein workflows with cleanup, validation, and downloads
Extract sequence features (CDS, mRNA, gene, etc.) from GenBank files in FASTA format with support for spliced features
Generate reverse, complement, or reverse-complement of DNA/RNA sequences
Translate DNA sequences to protein sequences using genetic code
Reverse translate protein sequences to possible DNA sequences
Convert CSV and TSV files containing sequence data to FASTA format with flexible column mapping and automatic delimiter detection
Convert FASTA sequence files to FASTQ format with mock quality scores
Convert standard FASTQ reads to FASTA with validation, IUPAC nucleotide support, average-quality filtering, and downloadable summaries
Convert GenBank files to FASTA format
Convert RNA sequences to DNA (reverse transcription) - replaces U with T
Convert TXT or plain text sequences into FASTA format files for DNA, RNA, and protein workflows with cleanup, validation, and downloads
Extract sequence features (CDS, mRNA, gene, etc.) from GenBank files in FASTA format with support for spliced features
Generate reverse, complement, or reverse-complement of DNA/RNA sequences
Translate DNA sequences to protein sequences using genetic code
Reverse translate protein sequences to possible DNA sequences
Configure inputs to begin
Set options on the left, then click “Convert”.
Transcription is the process by which cells copy DNA into RNA. In DNA, the four nucleotide bases are adenine (A), thymine (T), guanine (G), and cytosine (C). RNA uses uracil (U) instead of thymine—so transcription replaces every T with U while preserving the other bases.
This DNA to RNA converter performs that substitution: ATGC becomes AUGC.
Paste or upload DNA sequences, and the converter instantly produces the corresponding RNA. Processing happens in the browser with no server round-trip.
| Input | Description |
|---|---|
| DNA sequence | Raw sequence or FASTA format. Accepts .fasta, .fa, .fas, .seq, .txt files up to 50 MB. |
| Setting | Description |
|---|---|
Output case | UPPERCASE, lowercase, or preserve original case. Default: uppercase. |
Line wrapping | Wrap output at 60 or 80 characters per line, or output as a single line. Default: 80. |
Validate input sequences | Check that input contains only valid DNA bases (A, T, G, C, N). Enabled by default. |
Add suffix to headers | Append _rna to FASTA sequence headers. Enabled by default. |
RNA sequence(s) in the same format as the input. FASTA headers are preserved (with optional suffix).