RNA to DNA converter
Reverse transcribe RNA sequences to DNA by replacing uracil with thymine.
Input
Output
Configure input settings on the left, then click "Convert"
Reverse transcribe RNA sequences to DNA by replacing uracil with thymine.
Convert CSV and TSV files containing sequence data to FASTA format with flexible column mapping and automatic delimiter detection
Convert DNA sequences to RNA (transcription) - replaces T with U
Convert FASTA sequence files to FASTQ format with mock quality scores
Convert FASTQ sequence files to FASTA format
Convert GenBank files to FASTA format
Convert TXT or plain text sequences into FASTA format files for DNA, RNA, and protein workflows with cleanup, validation, and downloads
Extract sequence features (CDS, mRNA, gene, etc.) from GenBank files in FASTA format with support for spliced features
Generate reverse, complement, or reverse-complement of DNA/RNA sequences
Translate DNA sequences to protein sequences using genetic code
Reverse translate protein sequences to possible DNA sequences
Reverse transcription produces DNA from an RNA template. While normal transcription copies DNA to RNA (replacing T with U), reverse transcription does the opposite: uracil (U) becomes thymine (T).
This process occurs naturally via reverse transcriptase enzymes—most famously in retroviruses like HIV, and routinely in labs when creating cDNA from mRNA for sequencing or cloning.
The RNA to DNA converter performs the U→T substitution: AUGC becomes ATGC.
Enter RNA sequences in the browser for instant conversion—no software installation needed.
| Input | Description |
|---|---|
| RNA sequence | Raw sequence or FASTA format. Accepts .fasta, .fa, .fas, .seq, .txt files up to 50 MB. |
| Setting | Description |
|---|---|
Output case | UPPERCASE, lowercase, or preserve original case. Default: uppercase. |
Line wrapping | Wrap output at 60 or 80 characters per line, or output as a single line. Default: 80. |
Validate input sequences | Check that input contains only valid RNA bases (A, U, G, C, N). Enabled by default. |
Add suffix to headers | Append _dna to FASTA sequence headers. Enabled by default. |
DNA sequence(s) in the same format as the input. FASTA headers are preserved (with optional suffix).