RNA to DNA converter
Convert RNA sequences to DNA (reverse transcription). Replaces uracil (U) with thymine (T). Upload a FASTA file or paste your sequences below.
Convert RNA sequences to DNA (reverse transcription). Replaces uracil (U) with thymine (T). Upload a FASTA file or paste your sequences below.
Reverse transcription produces DNA from an RNA template. While normal transcription copies DNA to RNA (replacing T with U), reverse transcription does the opposite: uracil (U) becomes thymine (T).
This process occurs naturally via reverse transcriptase enzymes—most famously in retroviruses like HIV, and routinely in labs when creating cDNA from mRNA for sequencing or cloning.
The RNA to DNA converter performs the U→T substitution: AUGC becomes ATGC.
Enter RNA sequences in the browser for instant conversion—no software installation needed.
| Input | Description |
|---|---|
| RNA sequence | Raw sequence or FASTA format. Accepts .fasta, .fa, .fas, .seq, .txt files up to 50 MB. |
| Setting | Description |
|---|---|
Output case | UPPERCASE, lowercase, or preserve original case. Default: uppercase. |
Line wrapping | Wrap output at 60 or 80 characters per line, or output as a single line. Default: 80. |
Validate input sequences | Check that input contains only valid RNA bases (A, U, G, C, N). Enabled by default. |
Add suffix to headers | Append _dna to FASTA sequence headers. Enabled by default. |
DNA sequence(s) in the same format as the input. FASTA headers are preserved (with optional suffix).