Mutate DNA online and generate reproducible substitution, insertion, deletion, or mixed variant libraries from plain sequences or FASTA.
Generate random protein sequences with customizable length, composition, and amino acid properties
Generate random RNA sequences with customizable types and structural features
Shuffle DNA sequences while preserving nucleotide, dinucleotide, or k-mer composition for generating randomized control sequences
Convert CSV and TSV files containing sequence data to FASTA format with flexible column mapping and automatic delimiter detection
Translate DNA sequences to protein sequences using genetic code
Convert DNA sequences to RNA (transcription) - replaces T with U
Split FASTA files into record-based chunks by sequence count, target file count, maximum residues per file, or one file per sequence.
Convert FASTA sequence files to FASTQ format with mock quality scores
Convert standard FASTQ reads to FASTA with validation, IUPAC nucleotide support, average-quality filtering, and downloadable summaries
Mutate DNA online and generate reproducible substitution, insertion, deletion, or mixed variant libraries from plain sequences or FASTA.
Generate random protein sequences with customizable length, composition, and amino acid properties
Generate random RNA sequences with customizable types and structural features
Shuffle DNA sequences while preserving nucleotide, dinucleotide, or k-mer composition for generating randomized control sequences
Convert CSV and TSV files containing sequence data to FASTA format with flexible column mapping and automatic delimiter detection
Translate DNA sequences to protein sequences using genetic code
Convert DNA sequences to RNA (transcription) - replaces T with U
Split FASTA files into record-based chunks by sequence count, target file count, maximum residues per file, or one file per sequence.
Convert FASTA sequence files to FASTQ format with mock quality scores
Convert standard FASTQ reads to FASTA with validation, IUPAC nucleotide support, average-quality filtering, and downloadable summaries
Configure inputs to begin
Set options on the left, then click “Generate” — or start from an example.
Test sequences
High GC content
Low GC content
DNAGenIQ generates random DNA sequences with controllable parameters. Synthetic sequences serve as negative controls in sequence analysis, test datasets for bioinformatics pipelines, and placeholder inserts for cloning vector design.
ProteinIQ runs DNAGenIQ directly in the browser with instant results and no account required.
| Setting | Description |
|---|---|
Number of sequences | How many sequences to generate (default 1) |
Sequence length (bp) | Length of each sequence in base pairs (default 200) |
GC content (%) | Target percentage of G and C nucleotides (default 50%) |
Add restriction sites | Toggle to include enzyme recognition sequences |
Restriction sites | Select which site to add: EcoRI, BamHI, HindIII, PstI, XbaI, or SalI |
Sequences are returned in FASTA format with automatically generated headers. The output can be copied or downloaded directly.
The GC content parameter controls the ratio of guanine and cytosine to adenine and thymine. Natural DNA varies widely: bacterial genomes range from 25% to 75% GC, while mammalian genomes average around 40%. Setting GC content to 50% produces a balanced random sequence; lower values create AT-rich sequences, higher values create GC-rich sequences.
Restriction sites, when enabled, are inserted at the sequence termini to facilitate downstream cloning workflows.
Use DNAGenIQ when you need a new sequence with a chosen length and GC content. If you already have a DNA sequence and want controlled substitutions, insertions, deletions, or variant libraries, use the DNA mutation generator.