FASTA records with protein sequence identifiers.
Configure inputs to begin
Set options on the left, then click “Run CANYA” — or start from an example.
Two peptides

Predict protein solubility from amino acid sequence using the University of Manchester Protein-Sol method.

Predict multiple protein developability properties from amino-acid sequences using a multitask ProstT5 adapter.

Predict protein stability using validated BioPython methods: Instability Index, Aliphatic Index, GRAVY, flexibility analysis, and charge distribution

Predict protein thermostability changes (ΔΔG) for point mutations using a graph neural network. Enables computational saturation mutagenesis screening to identify stabilizing mutations.

Faithful static-mode Aggrescan3D tool for per-residue aggregation propensity analysis from a single protein structure.

Predict metal and water binding sites in protein structures using 3D convolutional neural networks (AllMetal3D + Water3D).

Predict pKa values of ionizable groups in proteins and protein-ligand complexes from 3D structure. PROPKA calculates environment-driven pKa shifts for standard ionizable residues, terminal groups, and supported ligand atom types.

Predict protein hydration sites from a structure using a diffusion model with ESM features and a confidence-filtering head.

Restore missing residues in antibody sequences using a language model trained on the Observed Antibody Space (OAS) database. Achieves better restoration than IMGT germlines or ESM-1b while being 7x faster.

Isoelectric Point Calculator 2.0 - Predict protein/peptide isoelectric point (pI) using 18+ validated pKa scales, SVR models, and deep learning. Supports proteins, peptides, and comprehensive analysis.