UniProt Download

Fetch UniProt protein sequence files, JSON records, and available sequence content by accession.

Input

Entry ID

Database

Output format

Output

Configure inputs to begin

Set options on the left, then click “Fetch downloads” — or start from an example.

P00533

P69905

Q8W3K0

How to download UniProt records?

UniProt Download fetches protein sequence files and JSON records from UniProt using a UniProt accession. It returns FASTA and JSON downloads, optional CIF structure links from cross-references, plus inline sequence content when the record includes a sequence.

Use this tool when you need a clean protein FASTA file for alignment, sequence analysis, structure prediction, or record keeping.

Input	Description
`Entry ID`	UniProt accession. Examples: `P00533`, `P69905`, `Q8W3K0`.

Setting	Description
`Database`	Opens the selected database download tool.
`Output format`	Return all files, FASTA, JSON, or CIF.

Supported UniProt downloads

File	Format	Use
FASTA	`.fasta`	Protein sequence record for analysis tools.
JSON	`.json`	UniProtKB record with identifiers, annotation, and metadata.
CIF	`.cif`	Structure file link when the UniProt record includes a mapped structure.
Protein sequence	`.fasta`	Inline FASTA generated from sequence content when available.

Understanding the results

The FASTA output is ready for sequence tools such as Protein Parameters, Amino Acid Composition, Clustal Omega, and ESMFold.

Filenames follow the pattern:

Text

uniprot-P00533-fasta.fasta
uniprot-P00533-json.json
uniprot-P00533-1m17.cif
uniprot-P00533-protein-sequence.fasta

The Details tab summarizes the accession, record title, file label, format, source type, filename, and URL.

Common use cases

Download a protein FASTA sequence before alignment.
Store a UniProt JSON record alongside analysis results.
Retrieve EGFR or hemoglobin sequences by accession.
Send a FASTA file to downstream sequence or structure tools.

FAQ

Should I use the accession or the gene name?

Use the accession for direct downloads. Gene names can refer to multiple proteins, isoforms, or organisms, so they are better suited to database search first.

Does this include reviewed and unreviewed entries?

The provider can retrieve UniProtKB records by accession. The record metadata indicates whether the entry is reviewed or unreviewed when that information is available.

Can I use the FASTA output for structure prediction?

Yes. The FASTA file can be used with structure prediction tools such as ESMFold, AlphaFold2, and Boltz-2.

Related tools

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InterPro Download

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PDBe Download

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QuickGO Download

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Analyze amino acid composition of protein sequences. The tool accepts FASTA sequences and outputs the percentage of each amino acid in the sequence.

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