Reverse complement converter

Generate reverse, complement, or reverse-complement of your DNA/RNA sequences. Upload a FASTA file or paste sequences below to convert them for opposite strand analysis or primer design.

Input (DNA/RNA sequences)

FASTA

Click to upload or drag and drop

FASTA file (.fasta, .fas, .fa, .txt)

Max size: 50MB

Conversion options

Operation type

Reverse complement

Both reverse and complement (5′→3′ antisense strand)

Complement only

A↔T, G↔C (same direction)

Reverse only

Reverse sequence order (3′→5′)

Additional options

Include original sequence in outputAdd operation labels to headersOutput RNA (T→U) instead of DNAValidate nucleotide charactersPreserve upper/lowercase

Operation examples:

Original: 5′-ATCG-3′

Reverse: 5′-GCTA-3′ (sequence reversed)

Complement: 5′-TAGC-3′ (bases complemented)

Reverse complement: 5′-CGAT-3′ (antisense strand)

💡 Reverse complement is most commonly used for finding genes on the opposite strand or designing primers.

Output (reverse complement sequences)

FASTA